Assay Genie have published an updated version of their 101 Western Blot Troubleshooting Tips Guide!

The Assay Genie Western Blot Troubleshooting Guide provides comprehensive solutions for common problems encountered during Western Blot experiments. 

It covers issues such as absent bands, poor transfer, high background, and weak signals. 

The guide stresses the importance of using specific antibodies, proper protein expression and extraction, correct electrophoresis, transfer techniques, and the use of a detectable marker on secondary antibodies.

Major sections of the guide include:

• General Western Blot Considerations

• Troubleshooting Non existant Bands

• Addressing Faint Bands or Weak Signals

• Resolving Non-Specific Bands

• Managing Incorrect Protein Band Sizes

• Improving Poor Protein Transfer

• Reducing High Background Issues

Finally, visit Assay Genie and choose from 1000s of Western blotting validated antibodies.