This kit is an excellent choice to detect changes in signalling pathways and gene regulation from cells transfected with reporter plasmids containing genes encoding Firefly and Renilla luciferases.
In the assay, Firefly luciferase is detected with Luciferin as a substrate and then Renilla luciferase is detected with coelenterazine as a substrate.
Usually, the transcriptional regulatory element is cloned upstream of Firefly luciferase, or the 3'-UTR regulatory region is cloned downstream of firefly luciferase.
The transfected cells are induced by a corresponding stimulator and lysed to determine the luciferase activity.
The stimulatory-inducing effect of the regulatory elements is evaluated by luciferase activity.
Renilla luciferase acts as an internal reference control for optimizing transfection efficiency and cell number.
Firefly luciferase catalyzes the emission of Luciferin at 560 nm and Renilla luciferase catalyzes the emission of coelenterazine at 465 nm.
This product is an economical alternative to other expensive Dual Luciferase assay kits
Disease Area Application
• Cancer: how cancer-realted genes or signaling pathways are activated
• Inflammation: measures activation of inflammatory pathways involved in diseases like rheumatoid arthritis
• Infectious diseases: how viral or bacterial infections affect host gene expression
• Neurodegenerative diseases: investigates gene activity to neuroprotection and neurodegeneration
• Metabolic disorders: studies gene regulation involved in diabetes and obesity
• Cardiovascular diseases: examines genes role in hearth disease development
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