| New Worthington Products for Nucleic Acid Research and Bioprocessing |
| • NUCxyme™ DNA/RNA Nuclease, Recombinant, AF |
| Serratia marcescens Nuclease |
| • Ribonuclease U2, Recombinant, Animal Free |
| Ustilago sphaerogena RNase U2 |
| • Protease Inhibitors, Animal Free |
| BBI, Kunitz, Lima Bean & Ovomucoid Inhibitors |
| Enzymes for Your Primary, Organoid and Single Cell Isolation Applications |
• Collagenases for Primary & Stem Cell Isolation
• STEMxyme Collagenase/Neutral Protease Blends
• Sequencing Proteases for Proteomics Research
• Papain for Neural Cell Isolation
• Papain for Neural, Endocrine Cells and Organoids
• Animal-free Nucleases, Proteases and Inhibitors
| Tissue Dissociation Guide Available |
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Worthington’s Tissue Dissociation Guide describes standard lab procedures; offers a logical experimental approach for establishing an optimal cell isolation protocol; and lists many tissue-specific references to aid development of an effective method. Available online, this technical guide covers cell isolation theory, cell isolation techniques and optimization strategies with additional resources. |
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| It is important to note, there is an area of optimized recovery balanced between yield and viability; working near the middle of this range will reduce variability in the results of the cell isolation procedure. Understanding this relationship and how it can vary with a particular cell type and application, can make the optimization process easier. For troubleshooting purposes various possible results, along with suggested corrective actions are listed below. Keep in mind that there are no clear lines between the quadrants but rather converging zones with variable areas of overlap. |
• Current Available Lots with Activity Profiles
• Interactive Collagenase Lot Selection Tool