Advanced Cell-Free Protein Synthesis System

PUREfrex® represents a breakthrough in cell-free protein synthesis technology, offering a precisely engineered in vitro coupled transcription/translation system. Unlike traditional S30 translation extracts, PUREfrex utilizes a completely reconstituted system derived from E. coli translation machinery. By adding DNA or mRNA that encodes the target protein to the reaction solution, proteins can be synthesized easily and quickly without using living cells.

Advantages of the PUREfrex protein synthesis system:

•  Protein expression in hours instead of days
•  Highly purified components eliminate contaminating nucleases & proteases
•  DNA and RNA template integrity preserved
•  Optimal protein functionality maintained
•  Maximum yield of full-length target proteins
•  Express proteins with any tag, including polyhistadine (HIS)
•  PUREfrex components can be customized to meet the expression demands of your unique protein 
•  PUREfrex volumes are easily scalable for protein production from µL to L quantities

Express Your Proteins in Hours, Not Days

PUREfrex® cell-free technology enables rapid, scalable protein production. Highly compatible with machine learning workflows.

Maximize Yields While Maintaining Protein Functionality

PUREfrex® Kits for Cell-free Protein Synthesis

PUREfrex® Reaction Supplements

Do you have a challenging protein to express? There is a variety of supplements available to optimize expression of your protein with the PUREfrex system.

PUREfrex® Custom Kit Development

For researchers with specialized protein expression needs, we offer the development of custom PUREfrex kits that are uniquely adapted to your project goals. 

Advantages of Custom PUREfrex:

•  Optimization of kit components for difficult-to-express proteins 
•  Addition of non-standard amino acids
•  Bulk sizing for large-scale reaction formats

Custom PUREfrex kit development includes consultation with technical experts to define your expression requirements, custom formulation of reaction components, and testing under controlled conditions to ensure you can produce high-yield, high-purity functional proteins. With rapid turnaround and flexible delivery formats, PUREfrex custom kits support your unique protein synthesis needs.

Get in touch with us to configure your custom kit!


Download PUREfrex Brochure


DBA-ITALIA Srl
Via Umbria 10
20054 Segrate (Milano)
Tel. +39 02-26922300

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