Traditional endotoxin testing uses horseshoe crab blood — an ancient and irreplaceable marine resource
ACROBiosystems offers a recombinant Factor C (rFC) solution that’s not only accurate, but also sustainable. Let’s protect these ancient creatures while ensuring your quality control!

SAFENSURE™ Recombinant Factor C Endotoxin Detection Kit
 

Assay Principles
 
The Recombinant Factor C Endotoxin Detection Kit uses recombinant technology to detect endotoxins by activating Factor C, which then cleaves a fluorogenic substrate to produce a measurable signal, as described in the original text. This fluorescent signal directly correlates with the endotoxin concentration, allowing for quantification on a white 96-well plate after a one-hour incubation at 37?. The measurement, conducted at 380/440 nm with a fluorescence reader, confirms contamination levels, as detailed further in.

Product Overview
 
The Recombinant Factor C Endotoxin Detection Kit is a novel endotoxin detection method based on the recombination technology. Recombinant Factor C, as the first component of the horseshoe crab coagulation cascade reaction, is activated by an endotoxin. The activated Factor C can cleave the fluorogenic substrate and produce a fluorescent signal. The increase of fluorescence signal is positively correlated with the dosage of endotoxin. The experiment is carried on a white 96-well plate and is measured at time zero and after a one-hour 37? incubation. Use a fluorescence microplate reader to measure at the wavelength of ex/em = 380/440 nm to determine whether the sample is contaminated by endotoxin.

 

Features, Advantages, Benefits
 
  1. Comparable to LAL method - Endpoint fluorescent assay, comparable to other chromogenic quantitative LAL methods.
  2. High specificity - Unlike LAL Assay, as Factor G is absent from the rFC test kit, false-positive results due to β-glucan activation are not expected to occur.
  3. Accuracy - Traceability of endotoxin standards in the kit against USP Standard (Catalog No: 1235503).
  4. Fast time to results - 1 hours.
  5. High sensitivity - Sensitivity range from 0.005-5 EU/mL.
  6. Extensive validation - Verification on multiplex biological products, kinds of microplate readers and various buffer systems, comprehensive validation of specificity, sensitivity, precision, accuracy, applicability, and other aspects according to the parameters listed in the EUROPEAN PHARMACOPOEIA 11.0, USP chapter<<1225>> and <86>.
  7. Sustainable resource - Get rid of dependence on animal derived reagents, reduce dependence on horseshoe crab resources and fishing pressure and realize long-term supply.
  8. Good inter batch consistency - Batch consistency of products is guaranteed due to the use of genetic recombination technology for production.

 

Materials Provided
 

Come and meet us in Fisciano!

 

DBA-ITALIA Srl
Via Umbria 10
20054 Segrate (Milano)
Tel. +39 02-26922300

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