As demand for AAV gene therapy vectors grows, so does the need for robust, quantitative analytics throughout the manufacturing process.

AAV Rep proteins drive genome replication and packaging, and their expression levels and isoform ratios are key indicators of producer cell performance. Monitoring Rep protein expression at defined production time points provides actionable insights into replication efficiency and helps identify the optimal harvest window.

Recombinant Rep Proteins available for AAV2 Rep40, Rep52, Rep68 and Rep78.

• Improve AAV Production: Monitor Rep40, Rep52, Rep68, and Rep78 protein expression during AAV manufacturing to optimize production.

• Understand your AAV Biology Better: Investigate genome replication, packaging efficiency, and integration mechanisms with a reference you can trust.

• Build your Setup: Order individual Rep proteins or the full recombinant set, whichever way suits your current process best.

• Flexible Use for Various Applications: Compatible with several applications, including western blot, capillary electrophoresis (CE), SDS PAGE, or Simple WesternTM technology (on Leo System™ from Bio-Techne).

• Easy-to-use: The liquid formulation is ready to use in your workflow.

• Off-the-shelf: In-stock and ready to ship, so your project timelines don't stall waiting for reagents.

Recombinant Replicase Protein Standards — The Value of Reliable Standards for AAV Vector Manufacturing and Quality Control

Quantitative Monitoring of AAV2 Rep Proteins Using Simple Western™ Enables Optimization of AAV Production Process