 |
Gangliosides are major constituents of neuronal cell membranes and endoplasmic
Reticulum and are involved in pathological states such as cancer, as certain distinctive gangliosides are found in tumors but not in the normal healthy tissue.
Indeed, they can be shed from the surface of tumor cells into the local environment, where they can influence interactions between cancer cells, including the transition of tumors from a dormant to a malignant state (angiogenesis).
The glycolipid asialo ganglioside-GM1 (ASGM1) is expressed by T cells in viral infection and by natural killer (NK) cells.
AS GM1 is localized in lipid raft structures in NK and CD8(+) T cells.
Anti Asialo GM1 antiserum has been shown to eliminate natural killer (NK) activity in cells of various strains of mice and rats.
Repeated immunization was performed with purified Asialo GM1 from bovine brain tissue, in conjunction with methylated bovine serum albumin and complete Freund‘s adjuvant. Gamma globulin fraction of serum was obtained by 50% ammonium sulfate precipitation methods followed by dialysis with phosphate buffered saline (pH 7.2).
Features
1. For Immunochemistry
2. Specific reactivity with mouse and rat NK cells, mouse monocytes and fetal thymocytes
Product Information
|
Description |
Wako Cat. No. |
Package Size |
Grade |
|
Anti Asialo GM1, (Rabbit) Ex |
986-10001 |
1 mL |
for Immunochemistry |
Product Specification
|
Immunoglobulin fractions |
Antibody Titer |
|
IgG, IgA, and IgM |
1:1000 by immunoflocculation test |
Specificity
|
Organism |
Cell Type/Tissue |
|
mouse and rat |
NK cells |
|
mouse |
monocytes (liver cells w/o NK cells), bone marrow; fetal liver cells, spleen cells of nude mice-macrophages |
|
mouse |
fetal |
References
1. Simons K, Ikonen E. Functional rafts in cell membranes. [Review]. Nature 1997; 387: 569±72.
2. Christie, W.W. and Han, X. Lipid Analysis - Isolation, Separation, Identification and Lipidomic Analysis (4th edition),
446 pages (Oily Press, Bridgwater, U.K. and Woodhead Publishing Ltd, Cambridge, U.K.)(2010).
3. Godney, E.K. and C.J. Gauntt: Journal of Immunology. 139, 913 (1987)
4. Habu, S., H. Fukui, M. Shimamura, M. Kasai, Y. Nagai, K. Okamura, and N. Tamaoki: Immunology. 127, 34 (1981).
5. Kasai, M., M. Iwamori, Y. Nagai, K. Okamura, and T. Tada: Eur. Journal of Immunology. 10, 175 (1980).
6. Okamura, K. and Y. Ochali: Metabolism. 17, 47 (1980).
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