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Live or Dead Cell viability and toxicity assays, designed for multiparameter experiments. AAT Bioquest.

Live or Dead™ Cell Viability Assays Optimized for Flow Cytometry

 


The effective labeling of cells provides a powerful method for studying cellular events in a spatial and temporal context.

One of the most common laboratory methods for determining cell viability is using cell-impermeant dyes. Compromised membranes of dead cells permit the influx of cell-impermeant dyes and become stained, whereas viable cells do not.

Each of the 10 unique Live or Dead™ Fixable Dead Cell Staining Kits employ membrane-impermeant amine-reactive dyes to differentiate live and dead cells during flow cytometry. Labeled cells can be fixed, permeabilized, and probed for intracellular immunophenotyping or other multiparameter staining experiments.

These kits are exceptional tools for preserving fluorescent images of particular cells and can also be used for fluorescence microscope demonstrations.

Investigating a unique aspect of cell viability? Not sure what materials you need? Contact us and our scientists can probably help.

Detection of Jurkat cell viability by Live or Dead™ Fixable Dead Cell Staining Kits. Jurkat cells were treated and stained, and then fixed in 3.7% formaldehyde and analyzed by flow cytometry. Live (Red), staurosporine treated (Green) and heat-treated (Blue) cells were distinguished with APC channel.Cell Viability Detection: Jurkat cells were treated and stained by Live or Dead™ Fixable Dead Cell Staining Kit (deep red), and then formaldehyde-fixed and analyzed by flow cytometry. Live (Red), staurosporine treated (Green) and heat-treated (Blue) cells were distinguished with APC channel.
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Live or Dead™ Cell Viability Assays

 

Resources & Information


AAT Bioquest has a wide range of resources and specialized products for researchers, including calculators, FAQs, & spectral profiles.

Related Digital Catalogs:
Cell Viability
Flow Cytometry Reagents

AssayWise Newsletter Research Article:
Multicolor Live or Dead™ Viability Assays for Flow Cytometry

Customer Favorites
Live or Dead™ Kits:
Fixable Dead Cell Staining Kits

Detection of Jurkat cell viability by Live or Dead™ Fixable Dead Cell Staining Kits (Cat#22602). Jurkat cells were heat- treated at 60oC or left untreated, and stained with Stain It™ Orange. Live and heat-treated cells were imaged with fluorescence microscope using TRITC filter.Cell Viability Imaging: Jurkat cells were heat- treated at 60°C or left untreated, then stained with Live or Dead™ Fixable Dead Cell Staining Kit. Live and heat-treated cells were imaged with fluorescence microscope using TRITC filter.

 

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