Cosmo Bio's Bone Resorption Activity Assay Kit is a multiwell solution for culture, differentiation and quantitative evaluation of bone resorption activity of osteoclasts.
The assay reports the fluorescence signal arising from the accumulation of fluoresceinamine-labeled chondroitin sulfate released from plate-bound calcium phosphate (carbonate apatite) into culture well media.

In this assay, fluorescienated chondroitin sulfate (a polyanion) bound to calcium phosphate coated cell culture wells releases to the culture supernatant in proportion to osteoclast resorption activity. Measurement of resorption activity is as simple as reading the fluorescence of a supernatant sample*.

* culture media without Phenol Red is recommended for optimal results

 Available in 24-, 48-, and new 96-well and 96-stripwell plates. Calcium phosphate coated plates and other assay components are also available separately.

Features:

• Time course of bone resorption activity easily obtained by measuring fluorescence of culture medium sample. 
• Well-established method utilized by researchers worldwide.  Imaging not required.
• Convenient CaP (hydroxyapatite) pre-coated wells; will not move within well.   
• Ready to use sterile components.
• Fluorescence excitation and emission wavelengths identical to FITC. NOTE: use culture media lacking Phenol Red.
• Permits microscopic observation of cell morphology.
• Permits pit area assay after cell removal _* using image analysis software.
• Suitable for evaluating osteoclast response to drugs such as bisphosphonates.

Bone Resorption Assay Flyer

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