FuGENE provides best-in-class transfection reagents that span applications
across DNA, RNA and CRISPR/Cas9 delivery, into a wide range of biologically relevant cell lines.

DNA Transfection:

Efficient, Gentle & Reliable

FuGENE 4K (4K-1000) is the newest and most advanced, 100% synthetic, multi-component transfection reagent designed for
the delivery of DNA into both challenging and routine mammalian cell lines:

• Efficient and safe entry of DNA into a wide range of cells
• Higher performance than other competitor reagents including Lipofectamine 3000
• Transfect difficult-to-transfect mammalian cell lines
• Transfect in serum-free or serum-containing media
• Improved viral titer and capsid fullness in AAV/LV systems
• Maximizes protein production in 293 & CHO cells

FuGENE4k quick protocol and technical manual.

Benefits of FuGENE 4k vs Lipofectamine 3000:

• PERFORMANCE: Obtain higher transfection efficiencies with FuGENE 4K compared to Lipofectamine 3000.
• EASE OF USE: FuGENE 4K is a single reagent kit, while Lipofectamine requires addition of separate reagent.
• CELL VIABILITY: FuGENE 4K has comparable cell viability as Lipofectamine across most cell lines.
• FASTER PROTOCOL: FuGENE 4K forms complex with DNA in as little as 5 minutes, and does not
  require additional component added to the transfection mix which requires preparation of an additional tube.
• COST SAVINGS: Less cost per mL of FuGENE 4K vs. Lipofectamine 3000. (at smallest available vial sizes)

RNA Transfection:

Superior RNA delivery with No Toxicity

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