CytoLiner Fixed Cell Membrane Dyes are novel lipophilic fluorescent dyes
developed specifically for selective plasma membrane staining in fixed cells for microscopy.
The dyes are engineered for robust and consistent staining of formaldehyde-fixed cells,
and are suitable for downstream immunofluorescence staining protocols.
Dyes:
CytoLiner 410/450, CytoLiner 495/510, CytoLiner 570/590,
CytoLiner 650/675, CytoLiner 680/710
Features:
- Robust and consistent staining of plasma membrane in formaldehyde-fixed cells
- Suitable for antibody co-staining
- Compatible with poly-L-lysine coated cultureware
- Available in 5 colors from blue to near-IR
Kit components:
-CytoLiner Fixed Cell Membrane Dye, 500X in DMSO
-CytoLiner Fixed Cell Staining Buffer, 100X
CytoLiner Dyes have optimized chemical structures to balance solubility with
lipophilicity to permit selective staining of the plasma membrane in fixed and mildly permeabilized cells.
CytoLiner staining is compatible with formaldehyde fixation and mild detergent permeabilization before staining.
Staining can tolerate blocking agents used during immunofluorescence staining protocols,
allowing subsequent staining with antibodies and other probes. The dyes also are compatible with poly-L-lysine
coated cultureware and Transwell permeable filter supports.
CytoLiner Dyes are available in a selection of 5 colors from blue to near-IR.
PFA-fixed MCF-7 cells were permeabilized with PBS/0.1% Triton X-100 for 10 minutes at room temperature,
then stained with 1X CytoLiner Fixed Cell Membrane Dye diluted in 1X CytoLiner Fixed Cell Staining Buffer
for 10 minutes at room temperature, followed by two rinses in PBS.
Cells were imaged in PBS by confocal microscopy. Scale bar: 20 um.
Suitable for Co-Staining with Antibodies and Other Probes
PFA-fixed MCF-7 cells were permeabilized with PBS/0.1% Triton X-100 for 10 minutes
at room temperature, then stained with 1X CytoLiner Fixed Cell
Membrane Dyes and NucSpot Nuclear Stains.
Click here and find the right stain for your application
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Membrane & Cell Surface Stains
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