PUREfrex® is a CosmoBio in in vitro coupled transcription/translation system, based on E. coli translation machinery.

By adding DNA or mRNA that encodes the target protein to the reaction solution, proteins can be synthesized easily and quickly without using living cells.

Purification with an advanced protocol, allow to obtain protein integrity and functionality.

Advantages for research processes:

• Expression of prokaryotic and eukaryotic proteins with high efficiency
• Synthesis of complex membrane proteins and potentially cytotoxic protein products
• Production of proteins containing disulfide bonds with native conformations
• Generation of site-specifically labeled proteins for analytical applications
• Incorporation of non-canonical amino acids for protein modification and engineering
• Expression of truncated protein variants for structure-function analysis and epitope identification

Advantages for protein expression processes:

• Verification and analysis of open reading frame (ORF) functionality
• Assessment of mutational effects on protein expression and ORF integrity
• Evaluation of amino acid substitutions on protein structure and function
• Implementation of ribosome display technology for protein evolution
• Investigation of fundamental translation mechanisms
• Advanced synthetic biology applications
• Protein folding mechanism studies
• Development of in vitro compartmentalization systems

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